Coding

Part:BBa_K3610010:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-08)


CORE ectodomain from S. lycopersicum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
    Illegal BamHI site found at 100
    Illegal BamHI site found at 1492
    Illegal BamHI site found at 1844
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The juxtamembrane domain must be included to ensure functional dimerization with the coreceptor BAk1.

Source

Cyril Zipfel Lab at UZH, Switzerland

References

Wang, Lei; Albert, Markus; Einig, Elias; Fürst, Ursula; Krust, Damaris; Felix, Georg (2016): The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein. In: Nature plants 2, S. 16185. DOI: 10.1038/nplants.2016.185.